TFS Autoloaders
Category: Sample preparation
Sub-category: Cryogenic work
Autoloader grid handling including loading, recovery, cassette care and nanocab care.
Knowledge of the workflow
Safety
Specific safety SOPs for this merit badge.
Relevant SDS (Safety Data Sheets)
Vendor product information
Autoloader compatible TEMs: TFS TEM product page.
Theoretical knowledge
- Overall workflow: Getting Started in CryoEM – Unit 2: Sample Preparation [Assembling autogrids, videos 21-24 of the unit 2 playlist]
- Overview of getting your grid into a microscope at CryoEM 101: https://cryoem101.org/chapter-3/#part2/
- Step by step instructions by Jonathan Ipsaro: Cryo-EM Sample Preparation: Part II – Grid Clipping
General SOP
A pass/fail test
The knowledge assessment test primarily will be given and may be updated regularly. If a user has earned the merit badge and policies have been updated, then an updated information sheet would have to be reviewed ahead of doing cryogenic work at the center.
Center staff training
The workflow demonstration should be scheduled with center staff through the NCCAT User Office or your appointed user coordinator.
Center staff training
The workflow demonstration should be scheduled with center staff through the NCCAT User Office or your appointed user coordinator.
Supervised and unsupervised practice by the user
Center staff will assist in arranging dedicated time slots for access to the necessary equipment. Registered users will be issued scheduler accounts through the NCCAT User Office.
Two (2) separate center staff observing your proficiency at separate times
Center staff will assist in arranging dedicated time slots for access to the necessary equipment. Appointments may be made through the NCCAT User Office.
Additional information:
Recertification period
- Sample preparation merit badges for cryogenic work are valid for ~6mo.
- Recertification (to maintain active status) would entail demonstrating proper use and care in front of a center staff member.
Furthering your knowledge
General background on plunge freezing
- Getting Started in CryoEM – Unit 2: Sample Preparation [Cryogenic work safety covered in the Plunge freezing safety modules]
