1.1 To transfer clipped autogrids into a TFS autoloader cassette and use a NanoCab to transfer them into an autoloader system
1.2 To care for and maintain autoloader tools (autoloader cassettes, transfer stations, and NanoCabs)
2.1 An Autoloader system is a robotic system for loading cryo-EM grids into the microscope, in which the user inserts autogrids into a cassette that is loaded into the microscope using a retractable arm.
2.2 An Autogrid is a Cryo-EM grid secured into a C-clip using clip rings (and prepared by the user through “grid clipping”).
2.3 A NanoCab is specially made vacuum insulated capsule that holds a cassette and docks with an Autoloader.
2.4 Liquid Nitrogen (LN2) is a cryogenic liquid stored under pressure.
2.5 Definition of terms for tools/equipment can be found in Figure 1.