Events

Workshops & courses

NCCAT

Details and registration on the NCCAT website

PNCC

Details and registration on the PNCC website

S2C2

Details and registration on the S2C2 website

CCET

  • Coming soon

MCCET

  • Coming soon

NCITU

  • Coming soon

SCSC

  • Coming soon

Talks & symposia

CryoEM Current Practices Webinar series

This monthly seminar series is jointly hosted by NCCAT, PNCC, & S2C2. Talks feature highlights and strategies from users of the centers with a heavy focus on cryoEM methods. Q&A after the seminar is meant to stimulate discussion of best practices and interesting challenges that will be helpful to researchers new to the field. Representatives from all three service centers will be on hand to answer questions about the cryoEM resources available to biomedical researchers and how to access them.

Talks take place on zoom the last Thursday of the month at 9 AM pacific/12 PM eastern and are are open to all at no-cost. Registration is required.

See our upcoming lineup and register via the links here:

  • 12/15/2022: A round-table discussion on single particle cryoEM data processing: “Particle picking with neural network pickers”
    Featuring a panel of national center staff members and developers of particle picking methods, names TBA.
    Register here

Past talks are available on our YouTube channel or can be viewed below.

Meet the CryoET Centers Webinar series

Cryo-electron tomography (cryo-ET) has revolutionized biological research by providing 3D reconstructions of biological structures with nanometer scale resolution. The NIH has funded a national network for cryo-electron tomography whose mission is to provide access to cryo-ET. Come join us for this webinar series on cryo-ET and learn about how you can access this network in support of your research.

Register here: https://uwmadison.zoom.us/webinar/register/WN_iRkuTJliRfOy1TA6tfNb8A

Webinar Topics:

  • An introduction to cryo-ET and overview of the National Network for Cryo-ET, July 8, 2021, Elizabeth R. Wright, MCCET
  • High pressure freezing, the waffle method and cryo-FIB, July 15, 2021, Mykhailo Kopylov, NCITU
  • Correlative cryogenic light and electron microscopy and tomography, July 22, 2021, Gong-Her Wu, SCSC
  • Preparation of select murine brain sections for HPF, cryo-CLEM, and cryo-ET, July 29, 2021, Michael H.B. Stowell, CCET
  • Structural studies of virus infection using CLEM, cryo-FIB, and cryo-ET, August 12, 2021, Jie E. Yang, MCCET

Previous webinars

Tips & tricks of note:

Sample preparation: Protein prep and grid freezing optimization, glutaraldehyde cross-linking

Data collection: Tilted data collection to overcome preferred orientations 

Data processing: Tips for processing tilted data, particle picking tips for tricky particles, helical filament processing 

A copy of this presentation is available on the Open Science Framework (OSF) repository: https://osf.io/x7dv8/  

Tips & tricks of note:

Data processing: computational infrastructure and resources

Tips & tricks of note:

Sample preparation: Optimization of grid freezing conditions  

Data processing: Tips for dynamic membrane proteins

Tips & tricks of note:

Sample preparation: Testing detergents and nanodisks for integral membrane proteins.

Data collection: Optimizing pixel size and data collection rate.

Data processing: Using symmetry during 3D reconstruction; cleaning particle stacks with 3D classification.

 

Tips & tricks of note:

Sample preparation: Sample QC using differential scanning calorimetry & negative stain EM. The effects of temperature on protein quality and grid preparation.

Other past events:

  • PNCC Data Processing Workshop – October 11-15, 2021
  • PNCC SerialEM Introductory Training – September 22-23, 2021
  • PNCC SerialEM Introductory Training – July 21-22, 2021
  • PNCC Data Processing Workshop – July 12-16, 2021
  • PNCC SerialEM Introductory Training – May 19-20, 2021
  • PNCC SerialEM Introductory Training – April 7-8, 2021
  • PNCC Data Processing Workshop – April 5-9, 2021
  • PNCC SerialEM Introductory Training – January 20-21, 2021
  • PNCC Data Processing Workshop – January 11-15, 2021
  • PNCC SerialEM Introductory Training – December 16-17, 2020
  • PNCC Data Processing Workshop – December 7-11, 2020
  • S²C² CryoEM CCP-EM Modeling Workshop – November 10-13, 2020
  • SSRL/LCLS2020 Stanford Synchrotron Radiation Lightsource (SSRL) and the Linac Coherent Light Source (LCLS) User’s Meeting: Cryo-EM of COVID-related Macromolecules – September 28, 2020
  • 2020 American Crystallographic Association (ACA) Annual Meeting: WK6 Fundamentals of Single Particle Cryo-EM – August 10-11, 2020
  • PNCC Data Processing Workshop – July 20-24, 2020
  • PNCC Data Processing Workshop – June 22-25, 2020
  • S²C² CryoEM Image Processing Workshop: cryoSPARC – June 10-12, 2020
  • Lecture with Dr. Yifan Cheng, HHMI UCSF (no presentation or recording available) & Dr. Christopher Booth, Amtek: Detection Technologies for Cryo-Electron Microscopy – May 29, 2020
  • Lecture with Dr. Robert Glaeser, LBNL: Cryo-specimen preparation, and principles of image phase contrast and phase plate – April 20, 2020
  • NCCAT Single Particle Short Course – March 2020
  • PNCC Sample Preparation Workshop – January 21-23, 2020
  • S²C² CryoEM Modeling Workshop: ChimeraX & Phenix – January 15-17, 2020
  • S²C² CryoEM Image Processing Workshop: SCIPION – November 18-20, 2019
  • S²C² CryoEM Training for Beginners Workshop – September 11-13, 2019
  • PNCC Microscope Operation Workshop – August 12-30, 2019
  • S²C² CryoEM Modeling Workshop: ChimeraX & Phenix – July 10-12, 2019
  • S²C² CryoEM Image Processing Workshop – March 11-13, 2019
  • S²C² CryoEM Training for Beginners Workshop – January 22-25, 2019