TFS Tundra TEM
Category: Microscope Operations
Sub-category: Microscope use and software control
Safety
You must complete all required safety training at the center you are visiting before hands on training. Coordinate this with the user office or training coordinator.
Background
Relevant Training Modules (to review until you can pass the knowledge test):
- TFS Tundra Resource Page
- Tundra Cryo-TEM virtual demonstration
- Review the basics of sample insertion and screening: CryoEM101 – Chapter 3
SOP for Tundra (also review before demonstration)
- Printable version of the SOP
- Digital version of the SOP
Vendor product information for reference
Additional reading:
The pass/fail knowledge assessment will test understanding of the following concepts:
- Tools needed to operate the Tundra
- Start up and shut down routines
- What is needed to perform alignments
Here is the current quiz version:
A workflow demonstration should be scheduled with center staff. The demonstration will cover all points of the SOP. You must have passed the knowledge quiz and reviewed the SOP before the demonstration.
Each center has additional specific Safety, Setup, and Shutdown procedures. Below, you will find those protocols for each center.
- NCCAT Site Specific Protcols – Tundra
- S2C2 Site Specific Protocols – Tundra
Schedule your supervised practice with the user office or appointed user coordinator. This can be scheduled for the same session as the demonstration. During supervised practice, center staff will be present for the full duration of the session to provide feedback and answer questions with the goal that you can pass the practical test for independence with no assistance. The number of supervised practice sessions will be tailored to the user, and if necessary, more than one session can be scheduled before taking the practical test.
Center staff will assist in arranging time for access to the necessary equipment and testing for independence. The practical test can not be scheduled during the same session as supervised practice.
A Tundra independence checklist will be used by the certifying staff to test for proper use of the instrument with a pass/fail outcome. All steps on the checklist must be performed to pass. You may use any instructions normally posted as a guide during the session.
Additional information:
Recertification period
- Microscope Operations merit badges are valid for ~4mo.
- Recertification (to maintain active status) would entail demonstrating proper use and care in front of a center staff member.